Primer análisis comparativo de la actividad antioxidante, hemolítica y antihemolítica de extractos acuosos de cinco especies del género hibiscus presentes en Latinoamérica
Date
Authors
Subject
fitoquímica
hemólisis
cálices
pétalos
phytochemistry
hemolysis
calyxes
petals
hemólisis
cálices
pétalos
phytochemistry
hemolysis
calyxes
petals
Language:
Journal Title
Journal ISSN
Volume Title
Publisher
Instituto Tecnológico de Santo Domingo (INTEC)
Introducción: El género Hibiscus pertenece a la familia Malvaceae y está compuesto por plantas herbáceas o arbustos que poseen flores solitarias, axila foliar y segmento del epicáliz lineares. Entre las numerosas especies del género Hibiscus la especie más conocida y estudiada es Hibiscus sabdariffa conocida como flor de Jamaica la cual se ha evidenciado que tiene una potente actividad antioxidante.
Objetivos: En la investigación se planteó como objetivo caracterizar y comparar la concentración de metabolitos biactivos y su actividad antioxidante, hemolítica y antihemolítica de extractos acuosos de cinco especies del género Hibiscus.
Materiales y métodos: los extractos se prepararon a partir de cálices de H. sabdariffa y pétalos deshidratados de las especies H. arnotiannus, H. cannabinus, H. mutabilis, H. rosa-sinensis. Para la actividad antioxidante se empleó el método de 2,2-difenil-1-picrilhidrazil (DPPH), inhibición de la oxidación de las LDL colesterol y una suspensión de glóbulos rojos.
Resultados: Las especies H. sabdariffa y H. cannabinus presentaron la mayor concentración de compuestos fenólicos y redujeron de manera significativa el DPPH. Así mismo se evidenció en los extractos puros de dichas especies que poseen una alta actividad hemolítica. Por otro lado las especies que exhibieron mejor actividad antihemolítica frente a peróxido fueron H. sabdariffa e H. cannabinus mientras que H. arnotiannus e H. mutabilis sus extractos puros inhibieron significativamente el efecto hemolítico del carbonato de calcio.
Conclusiones: los hallazgos permiten concluir que la actividad antioxidante, hemolítica y antihemolítica en estas especies se incrementa conforme aumenta el contenido de metabolitos bioactivos.
Introduction: The Hibiscus genus belongs to the Malvaceae family and is composed of herbaceous plants or shrubs that have solitary flowers, a linear leaf axil and segment of the epicalyx. Among the numerous species of the Hibiscus genus, the best known and studied species is Hibiscus sabdariffa, known as Jamaica flower, which has been shown to have powerful antioxidant activity. Objectives: The objective of the research was to characterize and compare the concentration of biactive metabolites and their antioxidant, hemolytic and antihemolytic activity of aqueous extracts of five species of the genus Hibiscus. Materials and methods: the extracts were prepared from calyxes of H. sabdariffa and dehydrated petals of the species H. arnotiannus, H. cannabinus, H. mutabilis, H. rosa-sinensis. For antioxidant activity, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was used, inhibition of the oxidation of LDL cholesterol and a suspension of red blood cells. Results: The species H. sabdariffa and H. cannabinus presented the highest concentration of phenolic compounds and significantly reduced DPPH. Likewise, it is evident in the pure extracts of these species that they have a high hemolytic activity. On the other hand, the species that exhibited the best antihemolytic activity against peroxide were H. sabdariffa and H. cannabinus, while H. arnotiannus and H. mutabilis, their pure extracts significantly inhibited the hemolytic effect of calcium carbonate. Conclusions: the findings allow us to conclude that the antioxidant, hemolytic and antihemolytic activity in these species increases as the content of bioactive metabolites.
Introduction: The Hibiscus genus belongs to the Malvaceae family and is composed of herbaceous plants or shrubs that have solitary flowers, a linear leaf axil and segment of the epicalyx. Among the numerous species of the Hibiscus genus, the best known and studied species is Hibiscus sabdariffa, known as Jamaica flower, which has been shown to have powerful antioxidant activity. Objectives: The objective of the research was to characterize and compare the concentration of biactive metabolites and their antioxidant, hemolytic and antihemolytic activity of aqueous extracts of five species of the genus Hibiscus. Materials and methods: the extracts were prepared from calyxes of H. sabdariffa and dehydrated petals of the species H. arnotiannus, H. cannabinus, H. mutabilis, H. rosa-sinensis. For antioxidant activity, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was used, inhibition of the oxidation of LDL cholesterol and a suspension of red blood cells. Results: The species H. sabdariffa and H. cannabinus presented the highest concentration of phenolic compounds and significantly reduced DPPH. Likewise, it is evident in the pure extracts of these species that they have a high hemolytic activity. On the other hand, the species that exhibited the best antihemolytic activity against peroxide were H. sabdariffa and H. cannabinus, while H. arnotiannus and H. mutabilis, their pure extracts significantly inhibited the hemolytic effect of calcium carbonate. Conclusions: the findings allow us to conclude that the antioxidant, hemolytic and antihemolytic activity in these species increases as the content of bioactive metabolites.
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info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artículos
info:eu-repo/semantics/publishedVersion
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Science, Environment and Climate; Vol. 6 No. 2 (2023): Science, Environment and Climate; 9-32
Ciencia, Ambiente y Clima; Vol. 6 Núm. 2 (2023): Ciencia, Ambiente y Clima; 9-32
2636-2333
2636-2317
10.22206/cac.2023.v6i2
Ciencia, Ambiente y Clima; Vol. 6 Núm. 2 (2023): Ciencia, Ambiente y Clima; 9-32
2636-2333
2636-2317
10.22206/cac.2023.v6i2