Tejidos fijados en formalina, embebidos en parafina (FFPE) como herramienta útil para extracción de ácidos nucleicos del SARS-CoV-2 en presuntas muertes causadas por COVID-19
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Date
Subject
SARS-CoV-2
FFPE tissues
postmortem
autopsy
COVID-19
SARS-CoV-2
tejido FFPE
post mortem
autopsia
COVID-19
FFPE tissues
postmortem
autopsy
COVID-19
SARS-CoV-2
tejido FFPE
post mortem
autopsia
COVID-19
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Journal Title
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Volume Title
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Intituto Tecnológico de Santo Domingo (INTEC)
En la República Dominicana, los primeros casos confirmados de Síndrome Respiratorio Agudo Severo asociado al Coronavirus tipo 2 (SARS-CoV-2) se notificaron a principios de marzo de 2020, con un aumento fluctuante entre diferentes hotspots en el territorio, y más de 500 mil casos con una tasa de letalidad que ha rondado entre 1.25-0.77 %. Debido al impacto del turismo en la economía dominicana, los primeros casos reportados en el país fueron asociados a visitantes de origen europeo. El objetivo de este estudio fue la detección post mortem del SARS-CoV-2 mediante la reacción cuantitativa en cadena de la polimerasa con transcripción inversa (qRT-PCR) en muestras de tejidos fijados con formalina e incluidos en parafina (FFPE) de muestras provenientes de casos sospechosos de la infección, y describir la relación con los hallazgos histopatológicos. Asimismo, evaluar el uso de qRT-PCR como herramienta para el diagnóstico post mortem de SARS-CoV-2 utilizando tejido FFPE, especialmente en aquellos casos en los que se desconoce la causa de muerte. Mediante un estudio retrospectivo realizado en un solo centro se seleccionaron dos casos que cumplían los criterios para el mismo. Los ácidos nucleicos se extrajeron de tejidos FFPE archivados seis meses después de las pautas internacionales de bioseguridad, por lo que se obtuvo ácido nucleico viral. El uso de tejidos FFPE podría ayudar a las autoridades de vigilancia epidemiológica a asignar un diagnóstico definitivo, principalmente en casos sospechosos de infecciones por SARS-CoV-2 y describir nuevos hallazgos histopatológicos de la enfermedad.
In the Dominican Republic, the first confirmed cases of severe acute respiratory syndrome associated with Coronavirus type 2 (SARS-CoV-2) were reported in early March 2020, with a fluctuating increase between different hotspots within the territory, and more than 500,000 cases with a fatality rate that has hovered between 1.25-0.77%. Due to the impact of tourism on the Dominican economy, the first cases reported in the country were associated with visitors of European origin. The aim of this study was the postmortem detection of SARS-CoV-2 by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in formalin-fixed paraffin-embedded (FFPE) tissue samples from of suspected cases of infection and describe the relationship with the histopathological findings. Likewise, to evaluate the use of qRT-PCR as a tool for the postmortem diagnosis of SARS-CoV-2 using FFPE tissue, especially in those cases in which the cause of death is unknown. Through a retrospective study carried out in a single center, two cases were selected that met the criteria. Nucleic acids were extracted from FFPE tissues archived six months due to international biosafety guidelines regulations, thus viral nucleic acid was obtained. The use of FFPE tissues could help epidemiological surveillance authorities assign a definitive diagnosis, mainly in suspected cases of SARS-CoV-2 infections and describe new histopathological findings of the disease.
In the Dominican Republic, the first confirmed cases of severe acute respiratory syndrome associated with Coronavirus type 2 (SARS-CoV-2) were reported in early March 2020, with a fluctuating increase between different hotspots within the territory, and more than 500,000 cases with a fatality rate that has hovered between 1.25-0.77%. Due to the impact of tourism on the Dominican economy, the first cases reported in the country were associated with visitors of European origin. The aim of this study was the postmortem detection of SARS-CoV-2 by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in formalin-fixed paraffin-embedded (FFPE) tissue samples from of suspected cases of infection and describe the relationship with the histopathological findings. Likewise, to evaluate the use of qRT-PCR as a tool for the postmortem diagnosis of SARS-CoV-2 using FFPE tissue, especially in those cases in which the cause of death is unknown. Through a retrospective study carried out in a single center, two cases were selected that met the criteria. Nucleic acids were extracted from FFPE tissues archived six months due to international biosafety guidelines regulations, thus viral nucleic acid was obtained. The use of FFPE tissues could help epidemiological surveillance authorities assign a definitive diagnosis, mainly in suspected cases of SARS-CoV-2 infections and describe new histopathological findings of the disease.
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info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/publishedVersion
Source
Science and Health; Vol. 6 No. 3 (2022): Science and Health, september-december; 35-43
Ciencia y Salud; Vol. 6 Núm. 3 (2022): Ciencia y Salud, septiembre-diciembre; 35-43
2613-8824
2613-8816
10.22206/cysa.2022.v6i3
Ciencia y Salud; Vol. 6 Núm. 3 (2022): Ciencia y Salud, septiembre-diciembre; 35-43
2613-8824
2613-8816
10.22206/cysa.2022.v6i3