Identificación molecular de Virus del Papiloma Humano de Alto Riesgo en carcinomas epidermoides: un piloto de implementación en entornos de recursos limitados
Files
Date
Subject
HPV infection
FFPE tissue samples
squamous cell carcinoma
nucleic acid amplification technique
cervical neoplasm
infección por VPH
muestras de tejido FFPE
carcinoma de células escamosas
técnica de amplificación de ácido nucleico
neoplasia de cuello uterino
FFPE tissue samples
squamous cell carcinoma
nucleic acid amplification technique
cervical neoplasm
infección por VPH
muestras de tejido FFPE
carcinoma de células escamosas
técnica de amplificación de ácido nucleico
neoplasia de cuello uterino
Language:
Journal Title
Journal ISSN
Volume Title
Publisher
Intituto Tecnológico de Santo Domingo (INTEC)
Introducción: el Virus del Papiloma Humano (VPH) es un factor importante en el desarrollo de cáncer cervical, cabeza y cuello. El VPH se clasifica en genotipos de bajo y alto riesgo, destacando estos últimos como un importante factor oncogénico. Las muestras fijadas en formalina y embebidas en parafina (FFPE) son una herramienta útil para identificación molecular del virus a través de la detección sistemática y la identificación de cada genotipo por separado de forma retrospectiva. El objetivo de esta investigación fue explorar un método de detección del VPH en FFPE en carcinomas de cérvix y en cabeza y cuello.
Materiales y métodos: se analizaron muestras preservadas en FFPE a través de ensayos de amplificación molecular Ampfire® HR HPV genotyping assay, para identificar 15 genotipos de alto riesgo y su potencial accesibilidad en entornos de bajos recursos. Se realizó un ensayo piloto en muestras con diagnóstico previo de carcinoma epidermoide en FFPE.
Resultados: se identificaron 12 casos con presencia de VPH. De estos, 9 de un total de 12 correspondieron al VPH16, las demás mostraron presencia de los genotipos VPH33, VPH39 y VPH52.
Conclusiones: las muestras de tejidos FFPE permitieron obtener muestras válidas para la identificación de VPH de alto riesgo. El ensayo estudiado demostró ser un buen candidato para la introducción de técnicas de genotipificación en tejidos FFPE de bajo costo en la República Dominicana.
Introduction: The Human Papilloma Virus (HPV) is an important factor in the development of cervical, head and neck cancer. HPV is classified into low-risk and high-risk genotypes, with the latter standing out as an important oncogenic factor. Formalin-fixed paraffin-embedded (FFPE) samples are a useful tool for molecular identification of the virus through systematic screening and identification of each separate genotype retrospectively. The objective of this research was to explore a method for detecting HPV in FFPE in carcinomas of the cervix and head and neck. Materials and methods: Samples preserved in FFPE were analyzed through molecular amplification assays Ampfire® HR HPV genotyping assay, to identify 15 high-risk genotypes and their potential accessibility in low-resource settings. A pilot trial was performed on samples previously diagnosed with squamous cell carcinoma in FFPE. Results: 12 cases with the presence of HPV were identified. Of these, 9 out of a total of 12 corresponded to HPV16, the others showed the presence of the HPV33, HPV39, and HPV52 genotypes. Conclusions: FFPE tissue samples allowed obtaining samples for the identification of valid high-risk HPV. The trial will study being a good candidate for the introduction of low-cost FFPE tissue genotyping techniques in the Dominican Republic.
Introduction: The Human Papilloma Virus (HPV) is an important factor in the development of cervical, head and neck cancer. HPV is classified into low-risk and high-risk genotypes, with the latter standing out as an important oncogenic factor. Formalin-fixed paraffin-embedded (FFPE) samples are a useful tool for molecular identification of the virus through systematic screening and identification of each separate genotype retrospectively. The objective of this research was to explore a method for detecting HPV in FFPE in carcinomas of the cervix and head and neck. Materials and methods: Samples preserved in FFPE were analyzed through molecular amplification assays Ampfire® HR HPV genotyping assay, to identify 15 high-risk genotypes and their potential accessibility in low-resource settings. A pilot trial was performed on samples previously diagnosed with squamous cell carcinoma in FFPE. Results: 12 cases with the presence of HPV were identified. Of these, 9 out of a total of 12 corresponded to HPV16, the others showed the presence of the HPV33, HPV39, and HPV52 genotypes. Conclusions: FFPE tissue samples allowed obtaining samples for the identification of valid high-risk HPV. The trial will study being a good candidate for the introduction of low-cost FFPE tissue genotyping techniques in the Dominican Republic.
Description
Type
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/publishedVersion
Source
Science and Health; Vol. 6 No. 3 (2022): Science and Health, september-december; 17-26
Ciencia y Salud; Vol. 6 Núm. 3 (2022): Ciencia y Salud, septiembre-diciembre; 17-26
2613-8824
2613-8816
10.22206/cysa.2022.v6i3
Ciencia y Salud; Vol. 6 Núm. 3 (2022): Ciencia y Salud, septiembre-diciembre; 17-26
2613-8824
2613-8816
10.22206/cysa.2022.v6i3